Chromatography practical.

During this lab session, we were tasked to separate a green food colouring into its components through chromatography.
Aim:

• To separate the green food colouring into the individual components.
• To determine the retention value (Rf value) of the separated components.

Apparatus:

• Boiling tube
• Strip of chromatography paper
• Boiling tube rack
• Capillary tube
• Clothes peg
• Hair dryer
• Green food colouring
• Distilled water

Procedure:

 

Diagram:

Calculating the retention value:

Observations: The food colouring is composed of blue dye and yellow dye. It is not a pure substance.

Image of chromatogram:

Results & Calculation of Rf values:

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